I’m reporting live from the Single Cell Genomics meeting, Oct 29 – Oct 31, at the Broad Institute in beautiful Cambridge, MA.
Unfortunately, the organisers discouraged the audience from live tweeting about the talks as this is considered a closed meeting. IMHO they’d have rather encouraged the speakers to first share their brand-new stuff on a preprint server (to be protected from being scooped) and then discuss it openly. I’ll thus only share information with prior permission from the speakers.
Mon, Oct 29
Shalin Naik: “SIS-seq, a molecular ‘time machine’, connects single cell fate with gene programs”
- dendritic cell (DC) development with discrete subsets of clones generating either cDC1, cDC2 xor pDC
- clonal history: split sister cells for different fate/sequencing analyses (bioRxiv 2018)
- multiple linear regression analysis revealed genes correlating with lineage bias
- mini pool CRISPR library to validate the influence of individual genes for identity of DCs
There was only a single talk I was allowed to share. I’ll reflect on this and, eventually, add some thoughts later on…